| Autor: |
Jun Takayama, Shuichi Onami |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Cell Reports, Vol 15, Iss 3, Pp 625-637 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2211-1247 |
| DOI: |
10.1016/j.celrep.2016.03.040 |
| Popis: |
Sperm induce Ca2+ waves in the fertilized egg by introducing soluble factors or by surface interactions, which activate egg Ca2+ channels. Involvement of sperm Ca2+ channels is predicted by the conduit model; however, this model has not been validated. In Caenorhabditis elegans, the sperm-specific TRP family Ca2+ channel TRP-3 mediates sperm-oocyte fusion. Here, using high-speed in vivo imaging and image analyses, we show that sperm induce an immediate local Ca2+ rise followed by a Ca2+ wave in fertilized C. elegans oocytes. Oocytes fertilized by rare trp-3 escaper sperm showed a lack of local rise and a delay in onset of the Ca2+ wave. Sperm Ca2+ imaging suggests that the local rise is not due to the bolus introduction of stored Ca2+. These results suggest that, along with its primary function in sperm-oocyte fusion, TRP-3 induces Ca2+ waves in fertilized oocytes, consistent with the conduit model. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
