Functionalized graphene grids with various charges for single-particle cryo-EM

Autor: Ye Lu, Nan Liu, Yongbo Liu, Liming Zheng, Junhao Yang, Jia Wang, Xia Jia, Qinru Zi, Hailin Peng, Yu Rao, Hong-Wei Wang
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nature Communications, Vol 13, Iss 1, Pp 1-8 (2022)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-022-34579-w
Popis: Air-water interface and preferential orientation problems are crucial challenges in cryo-EM specimen preparation. Here, the authors utilize graphene-coated EM grids functionalized by salts with various electrostatic properties, successfully overcoming preferred orientation.
Databáze: Directory of Open Access Journals