| Autor: |
N. Demeester, G. Castro, C. Desrumaux, C. De Geitere, J.C. Fruchart, P. Santens, E. Mulleners, S. Engelborghs, P.P. De Deyn, J. Vandekerckhove, M. Rosseneu, C. Labeur |
| Jazyk: |
angličtina |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 41, Iss 6, Pp 963-974 (2000) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)32039-3 |
| Popis: |
We investigated the lipoprotein distribution and composition in cerebrospinal fluid (CSF) in a group of patients with Alzheimer's disease (AD) or affected by other types of dementia in comparison to non-demented controls. We found slightly decreased apolipoprotein (apo)E and cholesterol concentrations in CSF of AD patients and moderately increased apoA-I concentrations, while in patients suffering from other types of dementia the apoA-I CSF concentration was increased. ApoA-IV concentrations varied widely in human CSF, but were not associated with any clinical condition. HDL2-like apoE-containing lipoproteins represent the major lipoprotein fraction. In CSF of normal controls, only a minor HDL3-like apoA-I-containing lipoprotein fraction was observed; this fraction was more prevalent in AD patients. ApoA-II was recovered mostly in the HDL3 density range, while apoA-IV was not associated with lipoproteins but appeared in a lipid-free form, co-localizing with LCAT immunoreactivity. Bi-dimensional analysis demonstrated pre-β and α apoA-I-containing particles; apoE and apoA-II were detected only in α-migrating particles. ApoA-IV distributed both to pre-β and γ-migrating particles; the LCAT signal was co-localized in this γ-migrating fraction. Enzymatically active LCAT was present in human CSF as well as PLTP activity and mass; no CETP mass was detected. In CSF from AD patients, LCAT activity was 50% lower than in CSF from normal controls.CSF lipoproteins induced a significant cholesterol efflux from cultured rat astrocytes, suggesting that they play an active role in maintaining the cholesterol homeostasis in brain cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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