Elevated levels of Merkel cell polyoma virus in the anophthalmic conjunctiva
| Autor: | Nora Siegal, Michal Gutowski, Lakshmi Akileswaran, Norman J. Beauchamp, Lien-Chieh Ding, Christopher B. Chambers, Russell N. Van Gelder |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2045-2322 01772961 |
| DOI: | 10.1038/s41598-021-92642-w |
| Popis: | Abstract The human ocular surface hosts a paucibacterial resident microbiome and virome. The factors contributing to homeostasis of this mucosal community are presently unknown. To determine the impact of ocular enucleation and prosthesis placement on the ocular surface microbiome, we sampled conjunctival swabs from 20 anophthalmic and 20 fellow-eye intact conjunctiva. DNA was extracted and subjected to quantitative 16S rDNA PCR, biome representational karyotyping (BRiSK), and quantitative PCR (qPCR) confirmation of specific organisms. 16S ribosomal qPCR revealed equivalent bacterial loads between conditions. Biome representational in silico karyotyping (BRiSK) demonstrated comparable bacterial fauna between anophthalmic and intact conjunctiva. Both torque teno virus and Merkel cell polyoma virus (MCPyV) were detected frequently in healthy and anophthalmic conjunctiva. By qPCR, MCPyV was detected in 19/20 anophthalmic samples compared with 5/20 fellow eyes. MCPyV copy number averaged 891 copies/ng in anophthalmic conjunctiva compared with 193 copies/ng in fellow eyes (p |
| Databáze: | Directory of Open Access Journals |
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