Doped Electrospinned Material-Guides High Efficiency Regional Bone Regeneration

Autor: Manuel Toledano, Cristina Vallecillo, María-Angeles Serrera-Figallo, Marta Vallecillo-Rivas, Aida Gutierrez-Corrales, Christopher D. Lynch, Manuel Toledano-Osorio
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Polymers, Vol 15, Iss 7, p 1726 (2023)
Druh dokumentu: article
ISSN: 2073-4360
DOI: 10.3390/polym15071726
Popis: The main target of bone tissue engineering is to design biomaterials that support bone regeneration and vascularization. Nanostructured membranes of (MMA)1-co-(HEMA)1/(MA)3-co-(HEA)2 loaded with 5% wt of SiO2-nanoparticles (Si-M) were doped with zinc (Zn-Si-M) or doxycycline (Dox-Si-M). Critical bone defects were effectuated on six New Zealand-bred rabbit skulls and then they were covered with the membranes. After six weeks, a histological analysis (toluidine blue technique) was employed to determine bone cell population as osteoblasts, osteoclasts, osteocytes, M1 and M2 macrophages and vasculature. Membranes covering the bone defect determined a higher count of bone cells and blood vessels than in the sham group at the top regions of the defect. Pro-inflammatory M1 appeared in a higher number in the top regions than in the bottom regions, when Si-M and Dox-Si-M were used. Samples treated with Dox-Si-M showed a higher amount of anti-inflammatory and pro-regenerative M2 macrophages. The M1/M2 ratio obtained its lowest value in the absence of membranes. On the top regions, osteoblasts were more abundant when using Si-M and Zn-Si-M. Osteoclasts were equally distributed at the central and lateral regions. The sham group and samples treated with Zn-Si-M attained a higher number of osteocytes at the top regions. A preferential osteoconductive, osteoinductive and angiogenic clinical environment was created in the vicinity of the membrane placed on critical bone defects.
Databáze: Directory of Open Access Journals
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