Comparison of the effect of three autogenous bone harvesting methods on cell viability in rabbits

Autor: Janet Moradi Haghgoo, Seyed Reza Arabi, Seyyed Mohammad Hosseinipanah, Ghasem Solgi, Neda Rastegarfard, Maryam Farhadian
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Journal of Dental Research, Dental Clinics, Dental Prospects, Vol 11, Iss 2, Pp 73-77 (2017)
Druh dokumentu: article
ISSN: 2008-210X
2008-2118
DOI: 10.15171/joddd.2017.014
Popis: Background. This study was designed to compare the viability of autogenous bone grafts, harvested using different methods, in order to determine the best harvesting technique with respect to more viable cells. Methods. In this animal experimental study, three harvesting methods, including manual instrument (chisel), rotary device and piezosurgery, were used for harvesting bone grafts from the lateral body of the mandible on the left and right sides of 10 rabbits. In each group, 20 bone samples were collected and their viability was assessed using MTS kit. Statistical analyses, including ANOVA and post hoc Tukey tests, were used for evaluating significant differences between the groups. Results. One-way ANOVA showed significant differences between all the groups (P=0.000). Data analysis using post hoc Tukey tests indicated that manual instrument and piezosurgery had no significant differences with regard to cell viability (P=0.749) and the cell viability in both groups was higher than that with the use of a rotary instrument (P=0.000). Conclusion. Autogenous bone grafts harvested with a manual instrument and piezosurgery had more viable cells in comparison to the bone chips harvested with a rotary device.
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