Slipknotted and unknotted monovalent cation-proton antiporters evolved from a common ancestor.

Autor: Vasilina Zayats, Agata P Perlinska, Aleksandra I Jarmolinska, Borys Jastrzebski, Stanislaw Dunin-Horkawicz, Joanna I Sulkowska
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: PLoS Computational Biology, Vol 17, Iss 10, p e1009502 (2021)
Druh dokumentu: article
ISSN: 1553-734X
1553-7358
DOI: 10.1371/journal.pcbi.1009502
Popis: While the slipknot topology in proteins has been known for over a decade, its evolutionary origin is still a mystery. We have identified a previously overlooked slipknot motif in a family of two-domain membrane transporters. Moreover, we found that these proteins are homologous to several families of unknotted membrane proteins. This allows us to directly investigate the evolution of the slipknot motif. Based on our comprehensive analysis of 17 distantly related protein families, we have found that slipknotted and unknotted proteins share a common structural motif. Furthermore, this motif is conserved on the sequential level as well. Our results suggest that, regardless of topology, the proteins we studied evolved from a common unknotted ancestor single domain protein. Our phylogenetic analysis suggests the presence of at least seven parallel evolutionary scenarios that led to the current diversity of proteins in question. The tools we have developed in the process can now be used to investigate the evolution of other repeated-domain proteins.
Databáze: Directory of Open Access Journals
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