Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

Autor: Olena S. Oliinyk, Anton A. Shemetov, Sergei Pletnev, Daria M. Shcherbakova, Vladislav V. Verkhusha
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-018-08050-8
Popis: Near-infrared (NIR) fluorescent proteins (FPs) offer advantages for spectral multiplexing and deep-tissue imaging. Here the authors engineer a smaller NIR FP based on the unexplored cyanobacteriochrome photoreceptor and demonstrate its use in various applications in cell culture as well as whole-body imaging in vivo in mice.
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