Added value of bleach for tuberculosis microscopy diagnostic in limited resources setting
| Autor: | Erike A. Suwarsono, Siti Nur Aisyah Jauharoh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Infectious Disease Reports, Vol 12, Iss 1s (2020) |
| Druh dokumentu: | article |
| ISSN: | 2036-7430 2036-7449 |
| DOI: | 10.4081/idr.2020.8719 |
| Popis: | Indonesia is one of the high burden tuberculosis (TB) countries. The utilization of molecular diagnostic has been setting over the country, however there are still a lot of remote areas that only depend on microscopic smear. Bleach might be used to increase the value of microscopy diagnos- tic. The study aimed to evaluate the added value of 1% bleach to increase the positivity rate of TB compared to conventional stain. This study was conducted on 70 subjects who were suspected as TB from various pri- mary care centers in West Java and Banten, Indonesia. The sputum was 2-3 mL, and divided into two parts, both of them were stained using Ziehl-Neelsen (ZN), however the first was only stained and the second added 1% bleach. Bleach was added in same volume with sputum and incubated for 10 minutes. After 10 minutes, the spu- tum was stained with ZN. The positivity rate for ZN only was 39 (55.7%) compared to 1% bleach was 63 (90%), and significant- ly different (P=0.000) using Mann-Whitney U test. Bleach can be used as an alternative agent to improve the TB smear in setting that only depends on microscopy diagnostic. |
| Databáze: | Directory of Open Access Journals |
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