Effects of putrescine, cadaverine, spermine, spermidine and β-phenylethylamine on cultured bovine mammary epithelial cells

Autor:	Kristen Sejrsen, Stig Purup, Eduard Ayuso, Raffaella Rebucci, Federica Cheli, Antonella Baldi, Eleonora Fusi
Jazyk:	angličtina
Rok vydání:	2010
Předmět:	Biogenic amines Cell cultures Bovine mammary gland. Animal culture SF1-1100
Zdroj:	Italian Journal of Animal Science, Vol 7, Iss 2, Pp 131-140 (2010)
Druh dokumentu:	article
ISSN:	1594-4077 1828-051X
DOI:	10.4081/ijas.2008.131
Popis:	A bovine mammary epithelial cell line (BME-UV1) and three-dimensional collagen primary bovine organoids were used to evaluate the effects of cadaverine, putrescine, spermine, spermidine and β-phenylethylamine on mammary epithelial cells. Each biogenic amine was diluted in several concentrations (0-50 mM in BME-UV1 and 0-4 mM in primary bovine organoids) in the appropriate saline solution for the cell culture considered. In order to determine the activity of each compound tritiated thymidine incorporation was used. At low concentrations, all amines induced cell proliferation in both cultures. In BME-UV1, spermine significantly inhibited cell proliferation (P
Databáze:	Directory of Open Access Journals
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