Effect of earthworm and papain enzyme concentration on antioxidant activity of earthworm (Eudrilus eugeniae) protein hydrolysate

Autor: Dianah Salsabila, Nur Hidayat, Suprayogi
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Advances in Food Science, Sustainable Agriculture, and Agroindustrial Engineering, Vol 6, Iss 4, Pp 362-373 (2023)
Druh dokumentu: article
ISSN: 2622-5921
DOI: 10.21776/ub.afssaae.2023.006.04.4
Popis: The earthworm Eudrilus eugeniae (African Night Crawler) can be used as a raw material for making protein hydrolysates. Hydrolysis of earthworms using papain enzyme produces peptides that have bioactivity as antioxidants. Antioxidant activity in earthworm hydrolysate is caused by phenolic compounds that have an important role in neutralizing free radicals. This study aims to determine the effect of earthworm concentration and papain enzyme concentration on soluble protein, total phenolic content, and antioxidant activity. This study used the Randomized Block Design (RBD) method arranged with two factors, namely earthworm concentration (20% 30%, and 40% (w/v)) and papain enzyme concentration (8%, 10%, and 12% (w/v)) with three replications. Data analysis used Analysis of Variance (ANOVA) and continued with the DMRT test. The results showed that earthworm concentration, papain enzyme concentration, and the interaction between the two factors had a significant effect (p
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