Study of glycine solution in preparation of frozen thawed deglycerolized erythrocytes
| Autor: | Daolin WANG, Hailong ZHUO, Hui LIU, Tingting CHEN, Qun LUO |
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| Jazyk: | čínština |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Zhongguo shuxue zazhi, Vol 36, Iss 7, Pp 583-586 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1004-549X 1004-549x |
| DOI: | 10.13303/j.cjbt.issn.1004-549x.2023.07.006&lang=en |
| Popis: | Objective To study the protective effect of glycine solution on frozen red blood cell thawing process. Methods A total of 20 bags of 1 U of leukocytes reduced suspended red blood cells within 6 days were selected for the study. After mixing, each 2 bags of suspended red blood cells were divided into 2 bags and into two groups with 10 bags of 1 U in each group, and were frozen for storage. One group was deglycerolized with sodium chloride solution (control group), and one group was deglycerolized with glycine solution (experimental group). The hemoglobin, free hemoglobin, residual glycerol, total glycerol in red blood cells, adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) were detected in the two groups. Results Compared with the free hemoglobin content (0.90±0.05)g/L and residual glycerol content (1.17± 0.08)g/L in the control group, the final product red blood cell supernatant free hemoglobin content (0.77±0.15)g/L and residual glycerol content (0.79±0.33)g/L in the experimental group were decreased, and the difference was statistically significant (P |
| Databáze: | Directory of Open Access Journals |
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