| Autor: |
Deivendran Rengaraj, Dong Gon Cha, Hong Jo Lee, Kyung Youn Lee, Yoon Ha Choi, Kyung Min Jung, Young Min Kim, Hee Jung Choi, Hyeon Jeong Choi, Eunhui Yoo, Seung Je Woo, Jin Se Park, Kyung Je Park, Jong Kyoung Kim, Jae Yong Han |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 1654-1669 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2001-0370 |
| DOI: |
10.1016/j.csbj.2022.03.040 |
| Popis: |
Avian germ cells can be distinguished by certain characteristics during development. On the basis of these characteristics, germ cells can be used for germline transmission. However, the dynamic transcriptional landscape of avian germ cells during development is unknown. Here, we used a novel germ-cell-tracing method to monitor and isolate chicken germ cells at different stages of development. We targeted the deleted in azoospermia like (DAZL) gene, a germ-cell-specific marker, to integrate a green fluorescent protein (GFP) reporter gene without affecting endogenous DAZL expression. The resulting transgenic chickens (DAZL::GFP) were used to uncover the dynamic transcriptional landscape of avian germ cells. Single-cell RNA sequencing of 4,752 male and 13,028 female DAZL::GFP germ cells isolated from embryonic day E2.5 to 1 week post-hatch identified sex-specific developmental stages (4 stages in male and 5 stages in female) and trajectories (apoptosis and meiosis paths in female) of chicken germ cells. The male and female trajectories were characterized by a gradual acquisition of stage-specific transcription factor activities. We also identified evolutionary conserved and species-specific gene expression programs during both chicken and human germ-cell development. Collectively, these novel analyses provide mechanistic insights into chicken germ-cell development. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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