Recognition Code of ZNF191(243-368) and Its Interaction with DNA

Autor:	Dongxin Zhao, Zhongxian Huang
Jazyk:	angličtina
Rok vydání:	2015
Předmět:	Biotechnology TP248.13-248.65 Inorganic chemistry QD146-197
Zdroj:	Bioinorganic Chemistry and Applications, Vol 2015 (2015)
Druh dokumentu:	article
ISSN:	1565-3633 1687-479X
DOI:	10.1155/2015/416751
Popis:	ZNF191(243-368) is the C-terminal region of ZNF191 which contains a putative DNA-binding domain of four Cys2His2 zinc finger motifs. In this study, an expression vector of a fusion protein of ZNF191(243-368) with glutathione-S-transferase (GST) was constructed and transformed into Escherichia coli BL21. The fusion protein GST-ZNF191(243-368) was expressed using this vector to investigate the protein-DNA binding reaction through an affinity selection strategy on the basis of the binding quality of the zinc finger domain. Results showed that ZNF191(243-368) can selectively bind with sequences and react with genes which contain an AGGG core. However, the recognition mechanism of Cys2His2 zinc finger proteins to DNA warrants further investigation.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/b886b25f7c2b41b39dcc103305c2a266 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF