Autor: |
Pablo Serrano-Lorenzo, Dino Gobelli, Rocío Garrido-Moraga, María J Esteban-Amo, José R López-López, Antonio Orduña, Miguel A de la Fuente, Miguel A Martín, María Simarro |
Jazyk: |
angličtina |
Rok vydání: |
2023 |
Předmět: |
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Zdroj: |
PLoS ONE, Vol 18, Iss 9, p e0291442 (2023) |
Druh dokumentu: |
article |
ISSN: |
1932-6203 |
DOI: |
10.1371/journal.pone.0291442 |
Popis: |
Increasing evidence demonstrate that the electron transfer chain plays a critical role in controlling the effector functions of macrophages. In this work, we have generated a Ndufs4-/- murine macrophage cell lines. The Ndufs4 gene, which encodes a supernumerary subunit of complex I, is a mutational hotspot in Leigh syndrome patients. Ndufs4-/- macrophages showed decreased complex I activity, altered complex I assembly, and lower levels of maximal respiration and ATP production. These mitochondrial respiration alterations were associated with a shift towards a pro-inflammatory cytokine profile after lipopolysaccharide challenge and improved ability to phagocytose Gram-negative bacteria. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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