Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
| Autor: | Adeílton Brandão, Turan Urmenyi, Edson Rondinelli, Antonio Gonzalez, Antonio B de Miranda, Wim Degrave |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 92, Iss 6, Pp 863-866 (1997) |
| Druh dokumentu: | article |
| ISSN: | 1678-8060 0074-0276 |
| DOI: | 10.1590/S0074-02761997000600024 |
| Popis: | Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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