Low temperature preservation of bovine ovaries on in vitro development of oocytes
| Autor: | Sung Woo Kim, Min Su Kim, Chan-Lan Kim, Dongkyo Kim, Namtae Kim, Hwan-Hoo Seong |
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| Jazyk: | English<br />Korean |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Journal of Animal Reproduction and Biotechnology, Vol 31, Iss 3, Pp 185-190 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2671-4639 2671-4663 |
| DOI: | 10.12750/JET.2016.31.3.185 |
| Popis: | During the ovary preservation in low temperature, the cumulus oocyte complexes(COCs) lose their developmental competences after in vitro fertilization. We used phosphate-buffered saline (PBS) as a basic solutions of at various temperatures (25, 15 or 5 °C) and supplemented them with 1mM glucose and 0.5mM glutamine as a source of carbohydrate metabolites. After recovery of COCs and in vitro fertilization, a significantly higher number of oocytes developed into blastocysts. The developmental competence of embryos that were originated from ovaries preserved at 15 °C was increased compared to those of 25 or 5 °C. The maturation rate of oocytes was not differed between 24 and 36 h at 15 °C but showed lower than control group (71% versus 78%). in vitro-fertilized oocytes from ovaries stored at 25 °C for 24 h or at 5 °C for 24 h had a significantly decreased developmental potentials, but at 15 °C did not (27% versus 29% of blastocysts to develop into day 8). With these results, bovine ovaries can be preserved at 15 °C for 36 h without decreasing developmental capacity of in vitro-fertilized oocyte at least to the blastocyst stage. This information provides valuable information of preserving ovaries for embryo transfer or in vitro embryo production. |
| Databáze: | Directory of Open Access Journals |
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