Method development and validation of paracetamol drug by RP-HPLC
| Autor: | T.A. Phazna Devi, Aravind Setti, S. Srikanth, Sivaramaiah Nallapeta, Smita C. Pawar, J. Venkateshwara Rao |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Journal of Medical and Allied Sciences, Vol 3, Iss 1, Pp 08-14 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2231-1696 2231-170X |
| Popis: | A simple and reproducible method was developed for paracetamol by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Paracetamol was separated on C18 column [4.6x250mm, particle size 5μm], using ortho phosphoric acid buffer with pH of 3.5 at the UV detection of 207nm. Isocratic elution of acetonitrile (ACN) and water was used as a mobile phase with various ratios and flow rates, eventually 25:75 v/v ACN and water was being set with the flow rate of 1mL/min. The statistical validation parameters such as linearity, accuracy, precision, inter-day and intraday variation were checked, further the limit of detection and limit of quantification of paracetamol concentrations were found to be 120ng/mL and 360ng/mL. Recovery and assay studies of paracetamol were within 99 to 102% indicating that the pro-posed method can be adoptable for quality control analysis of paracetamol. |
| Databáze: | Directory of Open Access Journals |
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