The Diversity and Distribution of Viruses Associated with Culex annulirostris Mosquitoes from the Kimberley Region of Western Australia

Autor: Simon H. Williams, Avram Levy, Rachel A. Yates, Nilusha Somaweera, Peter J. Neville, Jay Nicholson, Michael D. A. Lindsay, John S. Mackenzie, Komal Jain, Allison Imrie, David W. Smith, W. Ian Lipkin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Viruses, Vol 12, Iss 7, p 717 (2020)
Druh dokumentu: article
ISSN: 1999-4915
DOI: 10.3390/v12070717
Popis: Metagenomics revealed an impressive breadth of previously unrecognized viruses. Here, we report the virome of the Culex annulirostris Skuse mosquito, an important vector of pathogenic arboviruses in Australia. Mosquitoes were collected from three sites in the Kimberley region of Western Australia. Unbiased high-throughput sequencing (HTS) revealed the presence of 16 novel viral sequences that share less than 90% identity with known viruses. None were closely related to pathogenic arboviruses. Viruses were distributed unevenly across sites, indicating a heterogeneous Cx. annulirostris virome. Polymerase chain reaction assays confirmed HTS data and identified marked variation between the virus prevalence identified at each site.
Databáze: Directory of Open Access Journals
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