Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
| Autor: | Simone Sala, Yuhe Zhang, Nathaly De La Rosa, Till Dreier, Maik Kahnt, Max Langer, Lars B. Dahlin, Martin Bech, Pablo Villanueva-Perez, Sebastian Kalbfleisch |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Journal of Synchrotron Radiation, Vol 29, Iss 3, Pp 807-815 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1600-5775 16005775 |
| DOI: | 10.1107/S1600577522001874 |
| Popis: | X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view. |
| Databáze: | Directory of Open Access Journals |
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