Comparative Study on Molecular Weight of Konjac Glucomannan by Gel Permeation Chromatography-Laser Light Scattering-Refractive Index and Laser Light-Scattering Methods

Autor: Mei Xu, Dong-Sheng Li, Bin Li, Chao Wang, Yu-Peng Zhu, Wen-Ping Lv, Bi-Jun Xie
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Journal of Spectroscopy, Vol 2013 (2013)
Druh dokumentu: article
ISSN: 2314-4920
2314-4939
49909029
DOI: 10.1155/2013/685698
Popis: The gel permeation chromatography, combined with laser light scattering and refractive index (GPC-LLS-RI) online method and laser light scattering (LLS) single method, researched the weight-average molecular weight (Mw) of konjac glucomannan (KGM) in this paper. The results show that the determination results of molecular weight of KGM are similar by two method, the molecular weight of KGM is 2.476×105 g/mol by online method measured, and it is 2.508×105 g/mol by single method measured, compared with only 1.3% difference between the two methods. At the same time, the molecular weight distributions of KGM and conformation index were analyzed by online method, while the second virial coefficient (A2) that characterizes the interaction between KGM molecules and solvent molecules was acquired by single method, which can provide reliable foundation for the molecular chain morphology research of KGM.
Databáze: Directory of Open Access Journals
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