| Autor: |
Li Zhu, Siyan Li, Shikun He, Qizhe Tong, Lejin Wang, Xiaohua Li, Xi Wu, Qingyu Meng, Enzhong Jin, Chuan Zhang, Tianyuan Li, Ningda Xu, Lvzhen Huang, Yi Wang, Mingwei Zhao |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Eye and Vision, Vol 7, Iss 1, Pp 1-10 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2326-0254 |
| DOI: |
10.1186/s40662-020-00221-3 |
| Popis: |
Abstract Purpose To investigate the role of N6-methyladenosine (m6A) RNA modification in the pathogenesis of Graves' ophthalmopathy (GO). Methods Surgically excised extraocular muscles from 7 patients with GO and 5 subjects without GO were used. The global m6A levels in the specimens were determined using an m6A RNA methylation quantification kit. RNA sequencing (RNA-seq) was used to analyze the molecules involved in the regulation of m6A RNA methylation and the differential expression of mRNAs between the two groups (4 eyes, respectively). The expression of m6A RNA modification genes was evaluated by real-time PCR. The functional implications of the gene alterations between the GO and control specimens were determined by Gene Ontology analysis. Results The m6A level was significantly increased in the specimens of GO patients compared to the control specimens (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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