Endotracheal tube biofilm in critically ill patients during the COVID-19 pandemic : description of an underestimated microbiological compartment

Autor: Thomas Maldiney, Valentin Pineau, Catherine Neuwirth, Linda Ouzen, Isabelle Eberl, Géraldine Jeudy, Sophie Dalac, Lionel Piroth, Mathieu Blot, Marc Sautour, Frédéric Dalle, Caroline Abdulmalak, Romain Ter Schiphorst, Paul-Simon Pugliesi, Thomas Poussant, Agathe Ogier-Desserrey, Isabelle Fournel, Melchior de Giraud d’Agay, Marine Jacquier, Marie Labruyère, François Aptel, Jean-Baptiste Roudaut, Thibault Vieille, Pascal Andreu, Sébastien Prin, Pierre-Emmanuel Charles, Maël Hamet, Jean-Pierre Quenot
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-022-26560-w
Popis: Abstract Biofilm (BF) growth is believed to play a major role in the development of ventilator-associated pneumonia (VAP) in the intensive care unit. Despite concerted efforts to understand the potential implication of endotracheal tube (ETT)-BF dispersal, clinically relevant data are lacking to better characterize the impact of its mesostructure and microbiological singularity on the occurrence of VAP. We conducted a multicenter, retrospective observational study during the third wave of the COVID-19 pandemic, between March and May 2021. In total, 64 ETTs collected from 61 patients were included in the present BIOPAVIR study. Confocal microscopy acquisitions revealed two main morphological aspects of ETT-deposited BF: (1) a thin, continuous ribbon-shaped aspect, less likely monobacterial and predominantly associated with Enterobacter spp., Streptococcus pneumoniae or Viridans streptococci, and (2) a thicker, discontinuous, mushroom-shaped appearance, more likely characterized by the association of bacterial and fungal species in respiratory samples. The microbiological characterization of ETT-deposited BF found higher acquired resistance in more than 80% of analyzed BF phenotypes, compared to other colonization sites from the patient’s environment. These findings reveal BF as a singular microbiological compartment, and are of added clinical value, with a view to future ETT-deposited BF-based antimicrobial stewardship in critically ill patients. Trial registration NCT04926493. Retrospectively registered 15 June 2021.
Databáze: Directory of Open Access Journals
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