Identification of potential diagnostic biomarkers for hypertension via integrated analysis of gene expression and DNA methylation

Autor: Xiujiang Han, Jing Xue, Sheng Gao, Yongjian Li, Yuehe Duo, Feifei Gao
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Blood Pressure, Vol 33, Iss 1 (2024)
Druh dokumentu: article
ISSN: 08037051
1651-1999
0803-7051
DOI: 10.1080/08037051.2024.2387025
Popis: Objective Hypertension refers to the elevated blood pressure (BP) in arteries, with a BP reading of 140/90 mm Hg or higher in adults. Over 40% of >25-year-old population have suffered from hypertension. Thus, this study aimed to find novel diagnostic biomarkers for hypertension.Methods All hypertension-related mRNA and methylation datasets were downloaded from the GEO database. Liner model method was used to identify differentially expressed genes (DEGs) between hypertension and control groups. Gene Ontology and Kyoto Encyclopaedia of Genes and Genomes enrichment analysis was employed to obtain functional information. CpG sites and the corresponding genes associated with hypertension were screened using epigenome-wide association study (EWAS) analysis.Results There were 37 DEGs between the hypertension group and control group, which were significantly enriched in 84 Biological Process terms, 31 Cellular Component terms, 18 Molecular Function terms and 9 signalling pathways. EWAS results indicated that 1072 CpG sites were associated with hypertension occurrence, corresponding to 1029 genes. After cross-analysis, complement factor D (CFD) and OTU deubiquitinase, ubiquitin aldehyde binding 2 (OTUB2) with methylation modification were identified as diagnostic markers for hypertension.Conclusion In conclusion, CFD and OTUB2 were potential biomarkers of hypertension occurrence. Our results will provide more information for hypertension diagnosis and would be more reliable combined with multiple biomarkers.
Databáze: Directory of Open Access Journals
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