| Autor: |
Chunhui HAO, Wei CUI, Lingfei LUO, Yan LI |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Zhongguo gonggong weisheng, Vol 40, Iss 3, Pp 382-388 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1001-0580 |
| DOI: |
10.11847/zgggws1142965 |
| Popis: |
ObjectiveTo develop a fully automated method combining solid-phase extraction with ultra-high performance liquid chromatography tandem mass spectrometry for the determination of 52 types of antibiotics in drinking water. MethodsThe water samples were adjusted to pH 2 using phosphoric acid. The antibiotics in the water samples were enriched and purified through solid-phase extraction membrane. Subsequently, the samples underwent two elutions with 10 mL of 5% ammonia methanol, followed by nitrogen-assisted drying to near-dryness. Finally, the volume was adjusted to 1 mL using a 5% methanol aqueous solution for analysis purposes. Separation was achieved using an ACQUITY UPLC BEH C18 chromatographic column (2.1×100 mm, 1.7 μm). A mobile phase consisting of a gradient elution system comprising of 0.1% formic acid-water-0.1% formic acid methanol was employed for this purpose. Mass spectrometry utilized positive ion scan multiple reaction monitoring (MRM) mode. ResultsWithin the range of 0.1 – 100 μg/L, all regression equations obtained for the 52 target analytes exhibited satisfactory linear relationships. The method detection limit ranged from 0.1 to 0.6 ng/L, while the recovery rate varied between 65.1% and 133.3%. Additionally, the relative standard deviation (RSD) ranged from 3.1% to 20.5%. ConclusionThe established method is a simple, rapid, and highly accurate and precise approach for the simultaneous detection of 52 types of antibiotics in drinking water. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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