| Autor: |
Ulrich Dobramysl, Tom Beneke, Jeremy Charles Mottram, Carolina Moura Costa Catta-Preta, Richard Wheeler, Eva Gluenz |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Wellcome Open Research, Vol 7 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2398-502X |
| DOI: |
10.12688/wellcomeopenres.18575.1 |
| Popis: |
We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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