| Autor: |
Arun K. Bhunia, Tao Geng, Angela Valadez, Aparna Kothapalli, Byoung-Kwon Hahm, Daniel Ess, Mark T. Morgan, Giyoung Kim, Viswaprakash Nanduri |
| Jazyk: |
angličtina |
| Rok vydání: |
2006 |
| Předmět: |
|
| Zdroj: |
Sensors, Vol 6, Iss 8, Pp 808-822 (2006) |
| Druh dokumentu: |
article |
| ISSN: |
1424-8220 |
| DOI: |
10.3390/s6080808 |
| Popis: |
Recent outbreaks of food borne illnesses continue to support the need for rapidand sensitive methods for detection of foodborne pathogens. A method for detecting Listeriamonocytogenes in food samples was developed using an automated fiber-optic-basedimmunosensor, RAPTORTM. Detection of L. monocytogenes in phosphate buffered saline(PBS) was performed to evaluate both static and flow through antibody immobilizationmethods for capture antibodies in a sandwich assay. Subsequent detection in frankfurtersamples was conducted using a flow through immobilization system. A two stage blockingusing biotinylated bovine serum albumin (b-BSA) and BSA was effectively employed toreduce the non-specific binding. The sandwich assay using static or flow through mode ofantibody immobilization could detect 1 |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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