| Autor: |
Dimitrova Ivona, Bozhilova-Sakova Milena, Iliev Margarit, Teneva Atanaska |
| Jazyk: |
English<br />French |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
BIO Web of Conferences, Vol 42, p 01029 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2117-4458 |
| DOI: |
10.1051/bioconf/20224201029 |
| Popis: |
Karnobat sheep plays an important role in the development of sheep breeding in Southeastern region of Bulgaria. They are valuable source of genetic material. The aim of present experiment was to determine the allele variation of CAST gene in Local Karnobat and Karnobat Merino sheep breeds. A total of 60 blood samples were collected – 30 per breed. DNA was extracted and genotypes of all animals were identified by means of PCR-RFLP technique. The restriction reactions were accomplished by specific enzyme MspI. As expected both breeds were characterized with low level of genetic diversity due to the fact that mostly maintaining selection has been implemented. In Local Karnobat sheep breed was identified only one heterozygous individual from all 30. In Karnobat merino were identified allele M with frequency 0,97 and allele N with frequency 0,03. Genotypes MM and MN were revealed with frequencies 0,93 and 0,07, respectively. According to the statistical analysis both breeds were in HWE equilibrium. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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