| Autor: |
J Sadowska, M Kotula-Balak, B Bilinska |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
European Journal of Histochemistry, Vol 53, Iss 1, Pp 35-42 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1121-760X |
| DOI: |
10.4081/ejh.2009.35 |
| Popis: |
The aim of our study was to show whether the cells isolated from testes of patients underwent bilateral orchiectomy for prostatic cancer are able to grown in vitro, and if so, are functionally active. Immuncytochemistry was performed to show the functional status of human cultured cells. In detail, immunolocalization of luteinizing hormone receptors (LHR), mitochondria, and cytoskeletal elements was demonstrated. Moreover, radioimmunological assay was used to measure testosterone secretion by cultured Leydig cells. Using Nomarski interference contrast and fine immunofluorescence analysis the positive immunostaining for LHR was observed in almost all Leydig cells, however it was of various intensity in individual cells. Testosterone measurement revealed significant difference between testosterone secretion by hCG-stimulated and unstimulated Leydig cells (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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