Improved transfer efficiency of supercharged 36 + GFP protein mediate nucleic acid delivery
| Autor: | Lidan Wang, Jingping Geng, Linlin Chen, Xiangli Guo, Tao Wang, Yanfen Fang, Bonn Belingon, Jiao Wu, Manman Li, Ying Zhan, Wendou Shang, Yingying Wan, Xuemei Feng, Xianghui Li, Hu Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Drug Delivery, Vol 29, Iss 1, Pp 386-398 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1071-7544 1521-0464 10717544 |
| DOI: | 10.1080/10717544.2022.2030430 |
| Popis: | The potential of nucleic acid therapeutics to treat diseases by targeting specific cells has resulted in its increasing number of uses in clinical settings. However, the major challenge is to deliver bio-macromolecules into target cells and/or subcellular locations of interest ahead in the development of delivery systems. Although, supercharged residues replaced protein 36 + GFP can facilitate itself and cargoes delivery, its efficiency is still limited. Therefore, we combined our recent progress to further improve 36 + GFP based delivery efficiency. We found that the penetration efficacy of 36 + GFP protein was significantly improved by fusion with CPP-Dot1l or treatment with penetration enhancer dimethyl sulfoxide (DMSO) in vitro. After safely packaged with plasmid DNA, we found that the efficacy of in vitro and in vivo transfection mediated by 36 + GFP-Dot1l fusion protein is also significantly improved than 36 + GFP itself. Our findings illustrated that fusion with CPP-Dot1l or incubation with DMSO is an alternative way to synergically promote 36 + GFP mediated plasmid DNA delivery in vitro and in vivo. |
| Databáze: | Directory of Open Access Journals |
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