The Effect of Mesenchymal Stem Cell-Derived Microvesicles on Erythroid Differentiation of Umbilical Cord Blood-Derived CD34+ Cells

Autor: Davod Pashoutan Sarvar, Mohammad Hossein Karimi, Aliakbar Movassaghpour, Parvin Akbarzadehlaleh, Sara Aqmasheh, Hamze Timari, Karim Shamsasenjan
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Advanced Pharmaceutical Bulletin, Vol 8, Iss 2, Pp 291-296 (2018)
Druh dokumentu: article
ISSN: 2228-5881
2251-7308
DOI: 10.15171/apb.2018.034
Popis: Purpose: Mesenchymal stem cells (MSCs) play an important role in the proliferation and differentiation of hematopoietic stem cells (HSCs) in the bone marrow via cell-to-cell contact, as well as secretion of cytokines and microvesicles (MVs). In this study, we investigated the effect of mesenchymal stem cell-derived microvesicles (MSC-MVs) on erythroid differentiation of umbilical cord blood-derived CD34+ cells. Methods: In this descriptive study, CD34+ cells were cultured with mixture of SCF (10 ng/ml) and rhEPO (5 U/ml) cytokines in complete IMDM medium as positive control group. Then, in MV1- and MV2-groups, microvesicles at 10 and 20 µg/ml concentration were added. After 72 hours, erythroid specific markers (CD71 and CD235a) and genes (HBG1, GATA1, FOG1 and NFE2) were assessed by flow cytometry and qRT-PCR, respectively. Results: The expression of specific markers of the erythroid lineages (CD71 and GPA) in the presence of different concentration of microvesicles were lower than that of the control group (P
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