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Demiraw Bikila,1 Tadesse Lejisa,1 Yosef Tolcha,1 Chala Bashea,1 Mehari Meles,1 Tigist Getahun,1 Genet Ashebir,1 Wossene Habtu,1 Feyissa Challa,1 Ousman Mohammed,2 Melkitu Kassaw,3 Adisu Kebede,4 Letebrhan G/Egzeabher,5 Endalkachew Befekadu,6 Mistire Wolde,7 Aster Tsegaye7 1Department of National Clinical Chemistry Reference Laboratory, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 2Department of Medical Laboratory Sciences, Wollo University, Dessie, Ethiopia; 3Food Science and Nutrition Research Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 4National Capacity Building Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia; 5Department of Medical Laboratory, Yekatit 12 General Hospital, Addis Ababa, Ethiopia; 6Department of Medical Laboratory, Saint Amanuel Mental Specialized Hospital, Addis Ababa, Ethiopia; 7Department of Medical Laboratory Sciences, College of Health Science, Addis Ababa University, Addis Ababa, EthiopiaCorrespondence: Demiraw Bikila, Department of National Clinical Chemistry Reference Laboratory, Ethiopian Public Health Institute, Addis Ababa, Ethiopia, Email demiraw1234@gmail.comBackground: Even though several factors affect reference intervals (RIs), company-derived values are currently in use in many laboratories worldwide. However, few or no data are available regarding serum proteins RIs, especially in resource-limited countries such as Ethiopia.Objective: To establish RIs for serum protein electrophoresis of apparently healthy adults in Addis Ababa, Ethiopia.Methods: A cross-sectional study was conducted on a total of 297 apparently healthy adults from April to October 2019 in four selected sub-cities (Akaki, Kirkos, Arada, and Yeka) of Addis Ababa, Ethiopia. Laboratory analysis of collected samples was performed using the Capillarys 2 Flex Piercing analyzer, while statistical analysis was conducted using SPSS version 23 and Med-Calc software. The Mann–Whitney test was used to check partitions. A non-parametric method of reference range establishment was performed as per CLSI guideline EP28A3C.Results: The established RIs were: albumin 53.83– 64.59%, 52.24– 63.55%; alpha-1 globulin 3.04– 5.40%, 3.44– 5.60%; alpha-2 globulin 8.0– 12.67%, 8.44– 12.87%; and beta-1 globulin 5.01– 7.38%, 5.14– 7.86%. Moreover, the albumin to globulin ratios were 1.16– 1.8 and 1.09– 1.74 for males and females, respectively. The combined RIs for beta-2 globulin and gamma globulin were 2.54– 4.90% and 12.40– 21.66%, respectively.Conclusion: The established reference interval for serum protein fractions revealed gender-specific differences, except for beta-2 globulin and gamma globulin.Keywords: serum protein electrophoresis, reference interval, Addis Ababa, Ethiopia |
