Effect of osmotic pressure on spermatozoa characteristics of cryopreserved buffalo bull (Bubalus bubalis) semen

Autor:	Dawar Hameed Mughal, Ahmad Ijaz, Muhammad Shahbaz Yousaf, Fazal Wadood, Umer Farooq, Syed Aamer Mahmood, Amjad Riaz
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Buffalo bull semen quality osmotic pressure cryopreservation Veterinary medicine SF600-1100
Zdroj:	Journal of Applied Animal Research, Vol 46, Iss 1, Pp 274-277 (2018)
Druh dokumentu:	article
ISSN:	0971-2119 0974-1844 09712119
DOI:	10.1080/09712119.2017.1295971
Popis:	This study was designed to determine the effect of different osmotic pressures on spermatozoa characteristics of cryopreserved buffalo bull semen using tris egg yolk extender (TEYE). Semen of Nili-Ravi buffalo bulls (n = 4) housed at a semen production unit was collected at weekly intervals for 10 weeks. Three solutions of tris-citric acid-fructose with osmotic pressures of 255, 275 and 295 mOsm/kg were used in extender preparation. Semen straws containing 20 × 106 spermatozoa were processed and stored at −196°C in liquid nitrogen. Post-thaw analyses of spermatozoa included motility, viability, acrosomal integrity, plasma membrane integrity, DNA integrity and lipid per-oxidation. Significantly higher (P
Databáze:	Directory of Open Access Journals
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