Antibiotic resistance and molecular typing of clinical Staphylococcus aureus isolates from Malaysian military hospital

Autor: Nor Syaza Syahirah Amat Junaidi, Nik Noorul Shakira Mohamed Shakrin, Mohammad Fahmi Daman Huri, Ahmad Zakwan Kamarudin, Mohd Nasir Mohd Desa, Wan Mohd Zin Wan Yunus
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Asian Pacific Journal of Tropical Medicine, Vol 16, Iss 5, Pp 220-231 (2023)
Druh dokumentu: article
ISSN: 2352-4146
1995-7645
DOI: 10.4103/1995-7645.377743
Popis: Objective: To determine the antibiotic resistance profile (ARP) of Staphylococcus (S.) aureus isolates and molecular typing of the methicillin-resistant S. aureus (MRSA) isolates from Tuanku Mizan Armed Forces Hospital (TMAFH), Kuala Lumpur. Methods: The ARP and presence of the pvl gene were determined for 209 S. aureus isolates from clinical specimens. Of these, 123 were methicillin-susceptible S. aureus (MSSA) isolates and 86 were MRSA isolates. All MRSA isolates were characterized using SCCmec typing and spa typing. Descriptive analysis was performed to compare the demographic data with the phenotypic and genotypic variables of the S. aureus isolates. Results: No vancomycin-intermediate and -resistant S. aureus (VISA and VRSA, respectively) were detected among the study isolates. The MSSA isolates showed low resistance rates to all tested antibiotics, were commonly invasive (28/42, 66.7%), and mostly harboured pvl (35/42, 83.3%). Meanwhile, MRSA isolates showed high resistance to penicillin (86/86, 100%), ampicillin (86/86, 100%), sulbactam/ampicillin (86/86, 100%), cefuroxime (81/86, 94.19%), cefoperazone (76/86, 88.37%), azithromycin (56/86, 65.12%), and erythromycin (54/86, 62.79%). The majority of MRSA isolates were of SCCmec type IVh (65/86, 75.58%), spa type t032 (55/85, 63.95%), and grouped into spaCC-t022 (66/85, 77.65%). The t032 type was found to be associated with resistance traits to azithromycin and erythromycin (P
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