Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
| Autor: | Cláudia Carolina Silva Evangelista, Giovanna Vieira Guidelli, Gustavo Borges, Thais Fenz Araujo, Tiago Alves Jorge de Souza, Ubiraci Pereira da Costa Neves, Alan Tunnacliffe, Tiago Campos Pereira |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Iss 0 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1678-4685 49178210 |
| DOI: | 10.1590/1678-4685-gmb-2017-0030 |
| Popis: | Abstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|