| Autor: |
Alma Truyts, Ilse Du Preez, Eldas M. Maesela, Manfred R. Scriba, Les Baillie, Arwyn T. Jones, Kevin J. Land, Jan A. Verschoor, Yolandy Lemmer |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Tropical Medicine and Infectious Disease, Vol 9, Iss 11, p 269 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2414-6366 |
| DOI: |
10.3390/tropicalmed9110269 |
| Popis: |
Patient loss to follow-up caused by centralised and expensive diagnostics that are reliant on sputum is a major obstacle in the fight to end tuberculosis. An affordable, non-sputum biomarker-based, point-of-care deployable test is needed to address this. Serum antibodies binding the mycobacterial cell wall lipids, mycolic acids, have shown promise as biomarkers for active tuberculosis. However, anti-lipid antibodies are of low affinity, making them difficult to detect in a lateral flow immunoassay—a technology widely deployed at the point-of-care. Previously, recombinant monoclonal anti-mycolate antibodies were developed and applied to characterise the antigenicity of mycolic acid. We now demonstrate that these anti-mycolate antibodies specifically detect hexane extracts of mycobacteria. Secondary antibody-mediated detection was applied to detect the displacement of the monoclonal mycolate antibodies by the anti-mycolic acid antibodies present in tuberculosis-positive guinea pig and human serum samples. These data establish proof-of-concept for a novel lateral flow immunoassay for tuberculosis provisionally named MALIA—mycolate antibody lateral flow immunoassay. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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