Ginger extract modulates the expression of IL-12 and TGF-β in the central nervous system and serum of mice with experimental autoimmune encephalomyelitis
| Autor: | Abdollah Jafarzadeh, Rayhane Ahangar-Parvin, Maryam Nemati, Zahra Taghipour, Ali Shamsizadeh, Fatemeh Ayoobi, Zuhair Hassan |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Avicenna Journal of Phytomedicine, Vol 7, Iss 1, Pp 54-65 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2228-7930 2228-7949 |
| DOI: | 10.22038/ajp.2016.7002 |
| Popis: | Objective: The main function of IL-12 is differentiation of naive T cells intoTh1 cells and TGF-β is a powerful immunoregulatory cytokine. The immunomodulatory and anti-inflammatory properties of ginger have also been reported in some studies. The aim of this study was to evaluate the effects of ginger extract on the expression of IL-12 and TGF-β in a model of experimental autoimmune encephalomyelitis (EAE).Materials and Methods: EAE was induced in C57BL/6 mice by immunization with myelin oligodendroglial glycoprotein emulsified in complete Freund's adjuvant. The mice were administered intra-peritoneally with ginger extracts or PBS, from day +3 to +30. On day 31, mice were scarified and the expression of IL-12 and TGF-β mRNA in the spinal cord were determined by using real time-PCR. The serum levels of cytokines were measured by ELISA.Results: In PBS-treated EAE mice, the expression of IL-12 P35 and IL-12 P40 mRNA in the CNS and the mean serum levels of IL-12 were significantly higher than those of healthy group (p |
| Databáze: | Directory of Open Access Journals |
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