Microbial Diversity and Function in Shallow Subsurface Sediment and Oceanic Lithosphere of the Atlantis Massif

Autor: J. Goordial, T. D’Angelo, J. M. Labonté, N. J. Poulton, J. M. Brown, R. Stepanauskas, G. L. Früh-Green, B. N. Orcutt
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: mBio, Vol 12, Iss 4 (2021)
Druh dokumentu: article
ISSN: 2150-7511
DOI: 10.1128/mBio.00490-21
Popis: ABSTRACT The marine lithospheric subsurface is one of the largest biospheres on Earth; however, little is known about the identity and ecological function of microorganisms found in low abundance in this habitat, though these organisms impact global-scale biogeochemical cycling. Here, we describe the diversity and metabolic potential of sediment and endolithic (within rock) microbial communities found in ultrasmall amounts (101 to 104 cells cm−3) in the subsurface of the Atlantis Massif, an oceanic core complex on the Mid-Atlantic Ridge that was sampled on International Ocean Discovery Program (IODP) Expedition 357. This study used fluorescence-activated cell sorting (FACS) to enable the first amplicon, metagenomic, and single-cell genomic study of the shallow (
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