Comparative Metabolomic and Transcriptomic Analyses of Phytochemicals in Two Elite Sweet Potato Cultivars for Table Use

Autor: Lingxiao Zhao, Donglan Zhao, Shizhuo Xiao, An Zhang, Yitong Deng, Xibin Dai, Zhilin Zhou, Zhixian Ji, Qinghe Cao
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Molecules, Vol 27, Iss 24, p 8939 (2022)
Druh dokumentu: article
ISSN: 1420-3049
DOI: 10.3390/molecules27248939
Popis: To elucidate nutritional components in sweet potato cultivars for table use and to compare the phytochemicals of cultivars from different countries, ‘Kokei No. 14′ and ‘Xinxiang’ were selected. The physiological parameters and metabolites were determined using the colorimetric method and widely targeted metabolomics, respectively. Transcriptomic analysis was performed to explain the mechanism that resulted in phytochemical differences. ‘Xinxiang’ showed higher flavonoid and carotenoid contents. Metabolomics showed five upregulated flavonoids. Two essential amino acids (EAAs) and one conditionally essential amino acid (CEAA) were upregulated, whereas four EAAs and two CEAAs were downregulated. Unlike lipids, in which only one of thirty-nine was upregulated, nine of twenty-seven differentially accumulated phenolic acids were upregulated. Three of the eleven different alkaloids were upregulated. Similarly, eight organic acids were downregulated, with two upregulated. In addition, three of the seventeen different saccharides and alcohols were upregulated. In ‘other metabolites,’ unlike vitamin C, 6′-O-Glucosylaucubin and pantetheine were downregulated. The differentially accumulated metabolites were enriched to pathways of the biosynthesis of secondary metabolites, ABC transporters, and tyrosine metabolism, whereas the differentially expressed genes were mainly concentrated in the metabolic pathway, secondary metabolite biosynthesis, and transmembrane transport functions. These results will optimize the sweet potato market structure and enable a healthier diet for East Asian residents.
Databáze: Directory of Open Access Journals
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