Culture filtrate protein 32 as a potential target to attenuate the heterogeneous antibody response against Mycobacterium tuberculosis Antigens in different endemic settings

Autor: Chaouki Benabdessalem, Rym Ouni, Wafa Ben Hamouda, Jihene Bettaieb, Dahmani Mohamed Fathallah, Mohamed-Ridha Barbouche
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Mycobacteriology, Vol 11, Iss 4, Pp 378-383 (2022)
Druh dokumentu: article
ISSN: 2212-5531
2212-554X
DOI: 10.4103/ijmy.ijmy_127_22
Popis: Background: We previously reported the development of an enzyme-linked immunosorbent assay for the detection of the immunoglobulin G (IgG) response to Mycobacterium tuberculosis virulence factor – culture filtrate protein 32 (CFP32). The assay achieved high performance in comparing healthy Bacillus Calmette–Guerin-vaccinated controls with active tuberculosis (TB) patients from the Tunisian population. Herein, we aimed to assess the anti-CFP32 IgG response in suspected or confirmed active pulmonary TB individuals in different endemic settings. Methods: Serum samples were obtained from 224 donors from African and Latin American countries with variable levels of TB endemicity and different ethnical origins. Receiver operating characteristic curve was used to evaluate the performance of the serological assay. Results: The area under the curve was 0.70. The use of a cutoff level of 0.65 gave 67% and 68% sensitivity and specificity, respectively, regardless of ethnicity and endemicity. Except for the suspected Latin American group, overall multiple comparisons of medians pointed out the stability of the anti-CFP32 IgG response across the different endemic settings. Therefore, endemicity and ethnicity seem not to affect anti-CFP32 IgG response, mainly for detecting confirmed active TB individuals. Conclusions: These findings suggest that the inclusion of CFP32 epitopes in multi-antigen TB assay could attenuate serological differences related to heterogeneous endemicity and ethnicity. For this purpose, we further identified B-cell epitopes belonging to CFP32 by an in silico analysis.
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