A trypsin-like serine protease is involved in pseudorabies virus invasion through the basement membrane barrier of porcine nasal respiratory mucosa
| Autor: | Glorieux Sarah, Favoreel Herman W, Steukers Lennert, Vandekerckhove Annelies P, Nauwynck Hans J |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Veterinary Research, Vol 42, Iss 1, p 58 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1297-9716 0928-4249 |
| DOI: | 10.1186/1297-9716-42-58 |
| Popis: | Abstract Several alphaherpesviruses breach the basement membrane during mucosal invasion. In the present study, the role of proteases in this process was examined. The serine protease-specific inhibitor AEBSF inhibited penetration of the basement membrane by the porcine alphaherpesvirus pseudorabies virus (PRV) by 88.1% without affecting lateral spread. Inhibitors of aspartic-, cysteine-, and metalloproteases did not inhibit viral penetration of the basement membrane. Further analysis using the Soybean Type I-S trypsin inhibitor for the serine protease subcategory of trypsin-like serine proteases resulted in a 96.9% reduction in plaque depth underneath the basement membrane. These data reveal a role of a trypsin-like serine protease in PRV penetration of the basement membrane. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|