Evaluation of the effects of four media on human intestinal microbiota culture in vitro

Autor: Fu Yousi, Chen Kainan, Zhang Junnan, Xiao Chuanxing, Fan Lina, Zhang Bangzhou, Ren Jianlin, Fang Baishan
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: AMB Express, Vol 9, Iss 1, Pp 1-10 (2019)
Druh dokumentu: article
ISSN: 2191-0855
03969894
DOI: 10.1186/s13568-019-0790-9
Popis: Abstract The human intestinal microbiota has an important role in the maintenance of human health and disease pathogenesis. The aim of this research was to investigate the impact of four media on human intestinal microbiota metabolite and composition changes, we performed in vitro batch culture using intestinal microbiota samples from three fecal microbiota transplantation (FMT) donors. After 48 h culture, gut microbiota medium (GMM) had the highest production of acetic acid (73.00 ± 7.56 mM) and propionic acid (16.79 ± 1.59 mM), bacterial growth media (BGM) had the highest production of butyric acid (13.39 ± 0.56 mM). In addition, brain heart infusion (BHI) promoted (p
Databáze: Directory of Open Access Journals
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