An improved two dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives

Autor: B J H M Poorthuis, P J Yazaki, K Y Hostetler
Jazyk: angličtina
Rok vydání: 1976
Předmět:
Zdroj: Journal of Lipid Research, Vol 17, Iss 4, Pp 433-437 (1976)
Druh dokumentu: article
ISSN: 0022-2275
DOI: 10.1016/S0022-2275(20)34931-2
Popis: A two dimensional thin-layer chromatography system has been devised for the improved separation of phosphatidylglycerol and its derivatives, cardiolipin and bis(monoacylglyceryl)phosphate, from the other phospholipid components of tissue total lipid extracts. The system employs silica gel G plates prepared with 0.4 M boric acid. Linear recovery of added phosphatidylglycerol was found, and phosphatidylglycerol did not cochromatograph with N,N-dimethylphosphatidylethanolamine in this system. The phospholipid class composition of various rat tissues and a Morris 7777 hepatoma has been determined and compared with values from the literature.
Databáze: Directory of Open Access Journals