Comparative evaluation of antimicrobial efficacy of different combinations of calcium hydroxide against Enterococcus faecalis

Autor: Kavalipurapu Venkata Teja, Krishnamchari Janani, Kumar Chandan Srivastava, Deepti Shrivastava, Valentino Natoli, Marco Di Blasio, Macro Cicciu, Giuseppe Minervini
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: BMC Oral Health, Vol 23, Iss 1, Pp 1-10 (2023)
Druh dokumentu: article
ISSN: 1472-6831
DOI: 10.1186/s12903-023-03552-4
Popis: Abstract Background The study aims to compare the synergistic antibacterial efficacy of different combinations of calcium hydroxide as an intracanal medicament against E. faecalis. Material and methods The current study included four hundred extracted human permanent mandibular premolar teeth. After complete chemo-mechanical preparation, the middle third of the root was sectioned using a rotary diamond disc and a total of 400 samples were obtained. The specimens were inoculated with E. faecalis for 21 days. After that, specimens were divided into five groups (n = 80) based on materials used for the disinfection of samples: Group I, calcium hydroxide alone; Group II, calcium hydroxide + 2% chlorhexidine gel; Group III, calcium hydroxide + 2% chitosan gel; Group IV, calcium hydroxide + 0.02% silver nanoparticle gel; Group V, calcium hydroxide + Bioactive glass S53P4. Dentin shavings from the apical third were obtained from the inner third of dentin were obtained using gates glidden no.1 to the apical depth, followed by no.2, 3, 4 and 5 analyzed for E. faecalis using the culture method. One-way analysis of variance (ANOVA) was used for data analysis, followed by post-hoc Tukey's test for multiple comparisons of means to check the difference in bacterial inhibition between the groups. Results ANOVA results revealed a significant reduction of bacterial counts in all the groups compared (p
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