Evaluation of a commercial ELISA kit (IDEXX) to differentiate AI virus-infected poultry from AI-vaccinated poultry (DIVA)
| Autor: | F Talazadeh, M Mayahi, M Seifi, M Pourmehdi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Poultry Science, Vol 16, Iss 2, Pp 73-77 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1806-9061 1516-635x |
| DOI: | 10.1590/1516-635x160273-78 |
| Popis: | Vaccination programs for the control of avian influenza (AI) in poultry have limitations due to the problem of differentiating between vaccinated and virus-infected birds. The use of AI vaccination in poultry would have greater worldwide acceptance if reliable tests that clearly discriminated between naturally infected and vaccinated-only animals (DIVA) were available. In this study, to differentiate avian influenza virus (AIV)-infected chickens vs. chickens immunized with inactivated avian influenza virus, a commercial ELISA Kit (IDEXX) was used. Using HI as the reference method, the sensitivity of the commercial ELISA kit was 100% and its specificity was 100%. In the present study, we demonstrated that, 3 weeks after infection or vaccination, sera from both infected and vaccinated groups were tested positive and the difference in mean optical density between vaccinated and challenged birds, as detected by a commercial ELISA kit (IDEXX), was very small. Therefore, this assay cannot distinguish infected from vaccinated poultry. |
| Databáze: | Directory of Open Access Journals |
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