Sexually transmitted founder HIV-1 viruses are relatively resistant to Langerhans cell-mediated restriction.

Autor: Nina Hertoghs, Bernadien M Nijmeijer, Nienke H van Teijlingen, Angharad E Fenton-May, Tanja M Kaptein, John L van Hamme, John C Kappes, Neeltje A Kootstra, Beatrice H Hahn, Persephone Borrow, Carla M S Ribeiro, Teunis B H Geijtenbeek
Jazyk: angličtina
Rok vydání: 2019
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Zdroj: PLoS ONE, Vol 14, Iss 12, p e0226651 (2019)
Druh dokumentu: article
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0226651
Popis: A single HIV-1 variant establishes infection of the host after sexual contact. Identifying the phenotypic characteristics of these Transmitted Founder (T/F) viruses is important to understand the restriction mechanisms during transmission. Langerhans cells (LCs) are the mucosal dendritic cell subset that has been shown to have a protective role in HIV-1 transmission. Immature LCs efficiently capture and degrade HIV-1 via langerin-mediated restriction. Here we have investigated the capacity of T/F HIV-1 strains to infect mucosal Langerhans cells (LCs). Notably, most T/F variants efficiently infected immature LCs derived from skin and vaginal tissue in contrast to chronic HIV-1 laboratory strains. Next we screened a panel of T/F viruses and their matched 6-month consensus sequence viruses. Interestingly most T/F variants infected immature LCs whereas donor-matched 6-month consensus sequence viruses had lost the ability to infect LCs. However, we also identified 6-month consensus sequence viruses that had retained an ability to infect LCs similar to that of the donor-matched T/F virus. Moreover, some T/F viruses and 6-month consensus sequence viruses were unable to infect immature LCs. Further analyses indicated that T/F viruses are less sensitive to langerin-mediated restriction. These data suggest that T/F HIV-1 variants have the ability to infect immature LCs, which will facilitate transmission.
Databáze: Directory of Open Access Journals
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