Identificação de possíveis ESTs de Eucalyptus relacionados a genes responsáveis pelo alongamento celular (Nota Científica). Identification of possible ESTs of Eucalyptus related to genes responsible for elongation (Scientific Note)

Autor: Léo ZIMBACK, Edson Seizo MORI, Mario Luiz Teixeira de MORAES, Daniel Dias ROSA, Edson Luiz FURTADO, Celso Luiz MARINO, Ivan de Godoy MAIA, Carlos Frederico WILCKEN, Edivaldo Domingues VELINI, Iraê Amaral GUERRINI, Hideyo AOKI
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2012
Předmět:
Zdroj: Revista do Instituto Florestal, Vol 24, Iss 2, Pp 243-249 (2012)
Druh dokumentu: article
ISSN: 0103-2674
2178-5031
Popis: O objetivo deste estudo foi buscar in silico os ESTs do Banco de dados do Genoma de Eucalyptus (FORESTs, 2001) correlacionados com genes de alongamento celular envolvidos com crescimento de forma geral. Utilizando bibliotecas de cDNA, nós identificamos agrupamentos de ESTs semelhantes às proteínas controlando alongamento celular registradas no National Center of Biotechnologies Information – NCBI (2002). A busca mostrou similaridade para os seguintes agrupamentos de ESTs: o agrupamento EGEZLV2207D07.g com a enzima esterol delta redutase, EGEQRT4200A01.g com a proteína LKB biossintética de brassinosteróide, EGJMLV2220C06.g com EMBL-transportador mitocondrial half ABC, EGACST6260F07.g e EGMCFB1086B12.g com esterol 22 alfa hidroxilase (CYP90) (P450) e EGEQSL1051D09.g com EGEZSL5201E08.g para aproteína copine. The objective of study was in silico mining of ESTs from Eucalyptus ESTs database (FORESTs, 2001) correlated with cell elongation genes of growth character. Using cDNA libraries, we identified similar EST clusters to the proteins involved on control of cell elongation have been registered on National Center of Biotechnologies Information – NCBI (2002). The data mining has shown similarities for the following ESTs clusters: EGEZLV2207D07.g with delta sterol reductase, EGEQRT4200A01.g with LKB brassinosteroid biosynthetic protein, EGJMLV2220C06.g with EMBL-transporter of mitochondrial half ABC, EGACST6260F07.g and EGMCFB1086B12.g with steroid 22-α hydroxylase, and EGEQSL1051D09.g with EGEZSL5201E08.g for copine protein.
Databáze: Directory of Open Access Journals
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