| Autor: |
Xiaogang Huang, Zhihong Zhao, Ronggen Wang, Ying Ma, Yonghui Bu, Minhua Hu, Shouquan Zhang |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Veterinary Sciences, Vol 9, Iss 11, p 588 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2306-7381 |
| DOI: |
10.3390/vetsci9110588 |
| Popis: |
Procyanidin (PC) is a polyphenolic compound with antioxidant activity. The purpose of this study was to determine the influence of PC on canine sperm quality after 72 h of storage at 4 °C. The collected ejaculates were separated into four equal aliquots and treated with various concentrations of PC (0, 10, 30, and 50 μg/mL) in Tris-citric-fructose-egg yolk (TCFE) extender and stored at 4 °C for 72 h. The findings revealed that 30 μg/mL PC was the optimum concentration for significantly improving sperm motility (p < 0.05). Sperm samples treated with 30 μg/mL PC had substantially greater plasma membrane integrity, acrosome integrity, and mitochondrial membrane potential than the control group (p < 0.05). Furthermore, T-AOC and the expression levels of superoxide dismutase 1 (SOD1), catalase (CAT), and glutathione peroxidase 1 (GPx1) genes were significantly higher in sperm treated with 30 μg/mL PC than those in control (p < 0.05). In summary, this study discovered that adding PC to the TCFE extender enhanced sperm quality and that 30 μg/mL PC was the optimal concentration for canine sperm when stored at 4 °C. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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