| Autor: |
Alexandra M. Riding, Kevin W. Loudon, Andrew Guo, John R. Ferdinand, Laurence S.C. Lok, Nathan Richoz, Andrew Stewart, Tomas Castro-Dopico, Zewen Kelvin Tuong, Remi Fiancette, Georgina S. Bowyer, Aaron Fleming, Eleanor S. Gillman, Ondrej Suchanek, Krishnaa T. Mahbubani, Kourosh Saeb-Parsy, David Withers, Gordan Dougan, Simon Clare, Menna R. Clatworthy |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
iScience, Vol 25, Iss 7, Pp 104660- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2589-0042 |
| DOI: |
10.1016/j.isci.2022.104660 |
| Popis: |
Summary: Bladder infection affects a hundred million people annually, but our understanding of bladder immunity is incomplete. We found type 17 immune response genes among the most up-regulated networks in mouse bladder following uropathogenic Escherichia coli (UPEC) challenge. Intravital imaging revealed submucosal Rorc+ cells responsive to UPEC challenge, and we found increased Il17 and IL22 transcripts in wild-type and Rag2−/− mice, implicating group 3 innate lymphoid cells (ILC3s) as a source of these cytokines. NCR-positive and negative ILC3 subsets were identified in murine and human bladders, with local proliferation increasing IL17-producing ILC3s post infection. ILC3s made a more limited contribution to bladder IL22, with prominent early induction of IL22 evident in Th17 cells. Single-cell RNA sequencing revealed bladder NCR-negative ILC3s as the source of IL17 and identified putative ILC3-myeloid cell interactions, including via lymphotoxin-β-LTBR. Altogether, our data provide important insights into the orchestration and execution of type 17 immunity in bladder defense. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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