| Autor: |
ZHAO Lingyan, HE Liu, MA Yanni, YU Jia |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Jichu yixue yu linchuang, Vol 44, Iss 5, Pp 619-625 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1001-6325 |
| DOI: |
10.16352/j.issn.1001-6325.2024.05.0619 |
| Popis: |
Objective To investigate the impact of the RNA-binding protein with multiple splicing(RBPMS), which has multiple splicing functions, on the function of acute myeloid leukemia cells line THP-1 carrying the MLL-AF9 fusion gene. Methods The expression of RBPMS in the hematopoietic system was analyzed using the GEO database; RBPMS over-expression in THP-1 cells was achieved through lentiviral infection. The efficiency was detected by RT-qPCR and Western blot; The effect of RBPMS over-expression on the THP-1 cell transcriptome was evaluated by high-throughput sequencing. The impact of RBPMS over-expression on the proliferation and migration of leukemia cells was examined using CCK-8 assay and flow cytometry, respectively. Results RBPMS expressed in acute myeloid leukemia stem cells carrying the MLL-AF9 fusion gene was found to be inhibited. Over-expression of RBPMS significantly reduced the proliferation and migration of THP-1 cells. Conclusions RBPMS inhibits cell proliferation and migration of acute myeloid leukemia cells line carrying the MLL-AF9 fusion gene. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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